This is the blog for GW students taking Human Evolutionary Genetics. This site is for posting interesting tidbits on: the patterns and processes of human genetic variation;human origins and migration; molecular adaptations to environment, lifestyle and disease; ancient and forensic DNA analyses; and genealogical reconstructions.

GWHEG figure

GWHEG figure

Thursday, April 30, 2020

CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma

I think I forgot to post a potluck article during the CRISPR discussion week, so here is something interesting.

Long non-coding RNAs (lncRNA) have highly-specific expression in different cell types, giving them potential to be used for targeting specific types of cancer. However, very few lncRNAs have been studied or tested for these purposes yet.

Researchers in this paper used CRISPR interference to screen lncRNA loci on human glioblastoma cells. In class we talked about how CRISPR can be used to edit genomes, but it seems it can also be used to screen for gene function.

Through this method, they identified a number of lncRNA Glioma Radiation Sensitizers (lncGRS). Targeting of lncGRS inhibit growth of glioma cells (brain tumors), but not normal brain cells.



Zac Truesdell - CRISPR potluck/Journal update - Genome Biology

https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-01995-4

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